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doi: 10.1001/jama.2008.864 32 McDowellL

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A strong association between sgRNA and DNA further accelerates DNA strand separation until the PAM-proximal region forms a complete R loop, which instigates another conformational change in the HNH domain to induce the formation of DNA breaks by activating the nuclease activity of both RuvC and HNH domains [56, 57]

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