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Dead cells were detected either by trypan blue exclusion for experiments run in the presence of recombinant hGH (rhGH, Saizen ® , kindly provided by Serono France) at doses already experimented (Haeffner et al, 1999) or by flow cytometry analysis of cell lines after a 15 min staining with propidium iodide (PI) (5 μ g ml −1 pi, Sigma, St-Louis, MO, USA), using a FACScan (Becton Dickinson, Mountain View, CA, USA)