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Cells were kept in full medium containing high-glucose (4.5 g/l) DMEM (Gibco, 41966-029) supplemented with 10% fetal bovine serum (Gibco, 10500-064) and 1% penicillin/streptomycin (Gibco, 15140-122) in a humidified cell culture incubator (37°C, 5% CO 2 )