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While there have not been many studies conducted in iPSC-derived midbrain neurons that have examined the status of mitochondrial biogenesis compared to patient-derived fibroblasts, it is worth noting that a similar reduction in mitochondrial biogenesis - as evidenced by a significant reduction in mitochondrial content and PGC-1α mediated mitochondrial biogenesis, has been observed in Parkin-deficient iPSC-derived midbrain DA neurons from PD patients compared to their respective isogenic controls [89]

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The method, known as the R2KD assay, was used to characterise binding of NMR fragment screen hit 27 to TRF1 TRFH and revealed a K D value of 280 µM (95% confidence intervals (CI): 230–350 µM, Fig