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At the level of the regulatory mechanism of drug targets, the anti-TNF-α antibody adalimumab significantly inhibits the NF-κB and MAPK signaling cascade response by blocking the binding of TNF-α to the receptor, 542 whereas the ustekinumab antibody, which targets the IL-12/IL-23 axis, accurately inhibits the differentiation of Th1/Th17 cells and reduces the levels of IL-17 and IFN-γ by targeting its shared p40 subunit

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Real-time PCR Total RNAs were extracted using TRIZOL reagent (Thermo Fisher)